Coding
Part:BBa_K4275026:Design
Designed by: Yuxuan Bai Group: iGEM22_GreatBay_SCIE (2022-09-30)
Neae-Nb3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. The export tag situated on the N terminal of the Neae-Nb3 fusion construct directs the transporation of the target protein to the outer membrane of the bacteria from periplasm.
2. The beta-barrel domain (Figure 1) in the Neae region spans accross the outer memrbane of E.coli and provides structural support for the whole construct.
3. A spacer domain is situated between the C terminal Nb3 domain and the transmembrane beta-barrel domain.
Source
Escherichia coli (Neae)
Lama glama (Nb3)
References
1. Glass, David S, and Ingmar H Riedel-Kruse. “A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns.” Cell vol. 174,3 (2018): 649-658.e16. doi:10.1016/j.cell.2018.06.041